LOCUS       Exported                4026 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0049_producer_lasI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Daniel Gerngross
  TITLE     piG0049
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        50..84
                     /note="const. promoter (J23100)"
     RBS             93..104
                     /note="RBS (B0034)"
     CDS             111..719
                     /codon_start=1
                     /product="LasI (no LVA)"
                     /note="lasI (C0178)"
                     /translation="MIVQIGRREEFDKKLLGEMHKLRAQVFKERKGWDVSVIDEMEIDG
                     YDALSPYYMLIQEDTPEAQVFGCWRIFDTTGPYMLKNTFPELLHGKEAPCSPHIWELSR
                     FAINSGQKGSLGFSDCTLEAMRALARYSLQNDIQTLVTVTTVGVEKMMIRAGLDVSRFG
                     PHLKIGIERAVALRIELNAKTQIALYGGVLVEQRLAVS*"
     terminator      731..817
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      932..1703
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1704..2366
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            2377..2485
                     /note="incP origin of transfer"
     CDS             complement(2631..3821)
                     /note="TetR"
     terminator      3928..4022
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 actagtcgcc agggttttcc cagtcacgac gcggccgcaa gcttgcatgt tgacggctag
       61 ctcagtccta ggtacagtgc tagctactag tgaaagagga gaaatactag atgatcgttc
      121 agatcggtcg tcgtgaagag ttcgacaaaa aactgctggg tgaaatgcac aaactgcgtg
      181 ctcaggtttt caaagaacgt aaaggttggg acgtttccgt tatcgacgaa atggaaatcg
      241 acggttacga cgctctgtcc ccgtactaca tgctgatcca ggaagacacc ccggaagctc
      301 aggttttcgg ttgctggcgt atcttcgaca ccaccggtcc gtacatgctg aaaaacacct
      361 tcccggaact gctgcacggt aaagaagctc cgtgctcccc gcacatctgg gaactgtccc
      421 gtttcgctat caactccggt cagaaaggtt ccctgggttt ctccgactgc accctggaag
      481 ctatgcgtgc tctggctcgt tactccttgc agaacgacat ccagaccctg gttaccgtta
      541 ccaccgttgg tgttgaaaaa atgatgatcc gtgctggtct ggacgtttcc cgtttcggtc
      601 cgcacctgaa aatcggtatc gaacgtgctg ttgctctgcg tatcgaactg aacgctaaaa
      661 cccagatcgc tctgtacggt ggtgttctgg ttgaacagcg tctggctgtt tcctaataaa
      721 ttaaaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      781 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cagctgggcg
      841 cgccccccct acgggcttgc tctccgggct tcgccctgcg cggtcgctgc gctcccttgc
      901 cagcccgtgg atatgtggac gatggccgcg agcggccacc ggctggctcg cttcgctcgg
      961 cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca cgagcttgac
     1021 cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc tccaactgcg
     1081 cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag cctccggcct
     1141 gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc aaggctcgcg
     1201 cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc tatgcgagtg
     1261 ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc ggcgagtgcg
     1321 ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag tcgatcaaca
     1381 agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt gggggaaccc
     1441 cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat gcgaaagact
     1501 taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca ccccccgcaa
     1561 tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac gcaacgcata
     1621 attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca accgtgcggc
     1681 acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc attcaagcca
     1741 agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg tgggccgggc
     1801 ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg cagatgggcc
     1861 accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga cgttctttgc
     1921 ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc gtcgtgaagc
     1981 tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg tggggccagc
     2041 cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat cacgacgacc
     2101 aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg tatccgggcg
     2161 agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc attccgggca
     2221 tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc gggcagcagc
     2281 gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg gagccgccga
     2341 cacgggtaac gctgccgcgc cggtagggcc ggcctacggc cagcctcgca gagcaggatt
     2401 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
     2461 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
     2521 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
     2581 cgctataatg accccgaagc agggttatgc agcggaaaag gacaattgtc tcaggtcgag
     2641 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     2701 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
     2761 atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     2821 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     2881 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     2941 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
     3001 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     3061 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     3121 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     3181 gcggccacaa tggtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     3241 aagggcatcg gacggcgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     3301 aggttgaggc cgttgagcac cgccgccgca aggaatggtg cgtgcaggga gatggcgccc
     3361 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     3421 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     3481 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagagaat ccacaggacg
     3541 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     3601 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
     3661 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
     3721 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     3781 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     3841 tagcaattta actgtgataa actaccgcat tacagtttat cgatgataag ctgtcaaatt
     3901 taaatcgtaa ttattgggga cccctggatt ctcaccaata aaaaacgccc ggcggcaacc
     3961 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     4021 tccaag
//
